|Frances Arnold (left), George Smith (middle) and Gregory Winter (right) have won the 2018 Nobel Prize in chemistry|
Frances Arnold of Caltech becomes the fifth woman to win the Nobel Prize in chemistry. Her method of creating customized enzymes for biofuels, environmentally friendly detergents and other products won her The Prestigious Nobel Prize. Gregory Winter of the University of Cambridge and George Smith of the University of Missouri in Columbia were recognized for their development and use of a technique called phage display. This molecule-manufacturing process can generate biomolecules for new drugs. The trio will share the 9-million-Swedish-kronor prize with Arnold getting half and Winter and Smith splitting the other half.
A trio of researchers pioneered techniques that put natural evolution on fast-forward to build new proteins in the lab have earned three scientists this year’s Nobel Prize in chemistry. In the 1990s, Arnold wanted to make an enzyme that would break down a milk protein called casein in an organic liquid, rather than in water. Instead of trying to manually sculpt the chemical building blocks of that enzyme(subtilisin E) to give it the right properties, she opted for a more hands-off approach.
Arnold first made many copies of the original enzyme, each with a different set of genetic mutations. She then inserted the genes for those enzymes into bacteria. These bacteria served as living factories, churning out many copies of each enzyme variant. Arnold picked out the version that did the best job breaking down casein in an organic solvent and repeated the mutation process, starting with that enzyme.
After several rounds of mutating genes and choosing only the best enzyme to advance to the next round, Arnold was left with an extremely efficient custom-made enzyme. This enzyme-tailoring technique, known as directed evolution, allows scientists to fine-tune proteins the same way nature does, but thousands of times faster. Researchers have used directed evolution to create enzymes that jump-start chemical reactions for creating new drugs and eco-friendly biofuels. Arnold’s lab has also used directed evolution to create enzymes that help forge chemical connections not found in nature, such as bonds between carbon and silicon atoms.
The other half of the Nobel Prize honours work on a molecule-making procedure known as phage display. The primary tool for this process is a type of virus known as a bacteriophage a simple microbe made of genetic material enclosed in a protein package.
In the 1980s, Smith got the idea to insert the genes responsible for producing various specific proteins into bacteriophages’ genetic code, creating phages that bore these proteins on their surface. Smith’s original motivation was identifying which genes created which proteins. By fishing around a bacteriophage soup with a molecule known to bind to a certain protein, Smith surmised, a researcher could pick out only the phage armed with that particular protein and discover which gene allowed the phage to create it.
In 1985, Smith manipulated bacteriophage DNA to create a phage carrying a piece of protein, called a peptide, that wouldn’t naturally occur on its surface. He then used a peptide-binding molecule to pick this bacteriophage out of the crowd. Smith’s method of meddling with bacteriophage DNA is the foundation of phage display. Since his seminal work producing a peptide-bearing phage, other researchers have adopted his method to create phages that harbour other biomolecules, like antibodies.
Our bodies naturally produce hundreds of thousands of different antibodies that are designed to latch on to viruses and bacteria — essentially putting a hit on these invaders for the immune system to destroy. But researchers had long wanted to create in the lab antibodies that work as medications to curb various diseases. In 1990, Winter used the phage display method to create a phage armed with part of an antibody that binds to the molecule phOx. Winter then used phOx to collect the phage with the antibody from a collection of 4 million other phages.
To ensure that he was using phages to farm the best antibodies possible, Winter then adopted a similar method of directed evolution as Arnold. Winter first created a pool of bacteriophages genetically programmed to produce billions of different antibodies. From that group, he could use a target molecule, like phOx, to collect only the antibody-carrying phages that bound to it the best. From those phages, Winter created a new generation of antibody-toting viruses, and once again used the target molecule to pick out only the best of the bunch.
In the 1990s, Winter and his colleagues used this survival-of-the-fittest-phage technique to produce the antibody adalimumab, creating a drug that neutralizes a chemical that incites inflammation in patients with autoimmune diseases. The drug, known as Humira, was cleared to treat rheumatoid arthritis in 2002 and is now also used to treat psoriasis and inflammatory bowel disease.
These techniques could be used to create molecules that we haven’t yet discovered or have never even considered. It’s an open frontier.