Competition For Mitogens Regulates Spermatogenic Stem Cell Homeostasis In An Open Niche

The steady production of sperm relies on the number of sperm stem cells in the testis remaining constant. Researchers including Assistant Professor Yu Kitadate and Professor Shosei Yoshida (developmental biologists at the National Institute for Basic Biology within the National Institutes of Natural Sciences in Japan) and Professor Benjamin Simons (a theoretical physicist at the University of Cambridge in the U.K.) have revealed a novel mechanism for stem cell population control. Their results show that constant sperm stem cell numbers are achieved in mouse testes through a self-organized process in which they actively migrate and compete for a limited supply of self-renewal-promoting fibroblast growth factors (FGFs). This study was published on line in Cell Stem Cell.

To ensure a balance between the loss of differentiated cells and their replacement in long-lived multicellular organisms, it is critically important to keep the number of tissue stem cells constant. Failure to maintain stem cell number is thought to underlie the progression of aging and disease. In tissues like the testis and ovaries of the fruitfly Drosophila and intestine of mammalians, stem cells are clustered in their specialized home where self-renewal-promoting factors are abundant—this is the stem cell niche. In these tissues, stem cell numbers are controlled simply by the capacity of the niche. However, sperm stem cells are not clustered in mouse testis, but are highly motile and widely dispersed across the basement membrane. Yet, their density remains surprisingly uniform, thus raising the question of how their numbers are regulated.
In this study, the researchers found that a subset of lymphatic endothelial (LE) cells produce FGFs (Fgf5, 8 and 4 in particular), which promote stem cell self-renewal.

Assistant Professor Kitadate said, “The lymphatic endothelial cells in the testis were described via the use of electron microscopy in the 1970s, but had scant attention paid to them for a long time. By a stroke of good luck, our screening met these cells again and threw light on their hidden roles.”
Quantitative analyses of mice with increased or decreased FGF production revealed a simple mechanism: migratory stem cells uptake and consume FGFs. Stem cells which consume more FGFs are likely to duplicate, while those that consume less are inclined to differentiate. Under this framework, stem cells effectively compete with each other for a limited supply of FGFs, leading the stem cell number to automatically adjust to a particular value, depending on the rate of FGF supply. The discovery of a novel and extremely simple mechanism of stem cell number control based on “competition for self-renewal promoting factors (or mitogens)” advances the understanding of the regulation of stem cells in tissues without a canonical, anatomically definable stem cell niche—a microenvironment, sometimes called an open niche.

Professor Simons said, “As a general and robust mechanism of stem cell density control, these findings may have important implications for the regulation of stem cell density in other tissue types.”
Professor Yoshida said, “Sperm stem cells migrate in the testis to intake FGFs, just as cows move around the meadow to eat the grass which they live on. Interestingly, the dynamics of stem cells can be described using mathematics similar to that for ecosystem—a fruit of my beloved interdisciplinary research team!” Read:A fully Inkjet-Printed Disposable Glucose Sensor On Paper

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